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School of Biomedical Sciences
McClean, Stephen Dr
Senior Lecturer in Biomedical Sciences

Academic and Professional Qualifications
1994 - BSc (Hons) Science with Business, DIS (Commendation), University of Ulster
1996 - MRes (Distinction), University of Ulster
1999 - DPhil, University of Ulster
Career History
2001 - present: Lecturer in Proteomics, University of Ulster
1999 - 2001: Research Officer (Bioanalytical Chemistry), University of Ulster
Teaching & Learning Activities
Analytical chemistry, introductory physical chemistry, proteomic technologies
Main Research Interests
The isolation and characterisation of bioactive peptides and small molecules from amphibian skin secretions and reptile and arachnid venoms. This is done by QTOF MS/MS de novo sequencing of peptides, Edman degradation sequencing, FMOC solid phase peptide synthesis and MALDI-TOF mass spectrometry.
The quantitation of licit and illicit drug compounds in human hair samples by chromatographic and mass spectrometric methods is also of interest.
McClean, Stephen Dr - Contact details
|
Dr Stephen McClean |
Room Y031 |
Email: s.mcclean@ulster.ac.uk
McClean, Stephen Dr - Publications
McClean, Stephen, Hagan, William and Morgan, Jason (2011) Text messaging for student communication and voting. In: Effective Learning in the Biosciences, John McIntyre Conference Centre, Edinburgh, UK. Higher Education Academy UK Centre for Bioscience.
McClean, Stephen (2011) Inspiring the YouTube generation with reflective videos of practical classes. In: Effective Learning in the Biosciences, John McIntyre Conference Centre, Edinburgh, UK. Higher Education Academy UK Centre for Bioscience.
McClean, Stephen (2011) Investigation of Animal Venoms and Toxins. In: Methods in Animal Proteomics. Wiley-Blackwell, pp. 211-241. ISBN 978-0813817910
Mcclean, Stephen, Hagan, William and Morgan, Jason L. (2010) Text Messaging for Student Communication and Voting. Bioscience Education, 16.
McClean, Stephen and Hagan, William (2010) 'YouTestTube.com': using user-generated video to engage students. Perspectives on Pedagogy and Practice, 1. pp. 31-39.
Domann, Paula J., Akashi, Satoko, Barbas, Coral, Huang, Lihua, Lau, Wendy, Legido-Quigley, Cristina, McClean, Stephen, Neusuess, Christian, Perrett, David, Quaglia, Milena, Rapp, Erdmann, Smallshaw, Lance, Smith, Norman W., Smyth, Franklin and Taylor, Chris F. (2010) Guidelines for reporting the use of capillary electrophoresis in proteomics. NATURE BIOTECHNOLOGY, 28 (7). pp. 654-655.
Smyth, Thomas, Perfumo, A, McClean, Stephen, Marchant, Roger and Banat, Ibrahim (2010) Isolation and Analysis of Lipopeptides and High Molecular Weight Biosurfactants.. In: Handbook of Hydrocarbon and Lipid Microbiology. (Eds: Timmis, KN), Springer-Verlag, pp. 3688-3704. ISBN 978-3-540-77584-3
Moore, Sara, Smyth, Franklin, Gault, Victor, O'Kane, Edmund and McClean, Stephen (2009) Mass spectrometric characterisation and quantitation of selected low molecular mass compounds from the venom of Haplopelma lividum (Theraphosidae). RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 23 (12). pp. 1747-1755.
McClean, Stephen (2009) YouTestTube.com - video sharing to promote reflection in year one chemistry laboratory sessions. In: Science Learning and Teaching Conference 2009, Heriot-Watt University, Edinburgh. The Higher Education Academy Subject Centres for Bioscience, Materials and Physical.
Hagan, William, Ruddick, J David and McClean, Stephen (2008) Supporting first year chemistry in the biosciences. In: Variety in Chemistry Education, Dublin City University. The Higher Education Academy, UK Physical Sciences Centre.
MClean, Stephen (2008) Excel Yourself with Personalised Email Messages. UK Centre for Bioscience, 4 pp, DOI: http://dx.doi.org/10.3108/beej.12.c1
McClean, Stephen (2008) Making Small Talk — Audio MP3 Files Made More Portable. UK Centre for Bioscience, 1 pp
Thompson, Alan Hunter, Bjourson, AJ, Orr, David Francis, Shaw, Chris and McClean, Stephen (2007) Amphibian skin secretomics: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a novel peptide family, the hyposins. JOURNAL OF PROTEOME RESEARCH, 6 (9). pp. 3604-3613.
Thompson, Alan Hunter, Bjourson, AJ, Orr, David Francis, Shaw, Chris and McClean, Stephen (2007) A combined mass spectrometric and cDNA sequencing approach to the isolation and characterization of novel antimicrobial peptides from the skin secretions of Phyllomedusa hypochondrialis azurea. PEPTIDES, 28 (7). pp. 1331-1343.
Doherty, Bernadette, O'Donnell, Fionnuala, Smyth, Franklin, Leslie, Julian, Ramachandran, Venkataraman N, Boyd, Neil S, Hack, Catherine, O'Kane, Edmund and McClean, Stephen (2007) A study of the analytical behaviour of selected psycho-active drugs using liquid chromatography, ion trap mass spectrometry, gas chromatography and polarography and the construction of an appropriate database for drug characterisation. TALANTA, 72 (2). pp. 755-761.
Zhou, J. W., Bjourson, AJ, Coulter, Danny, Chen, T. B., Shaw, C., O'Rourke, M., Hirst, D. G., Zhang, Y. N., Rao, P. F. and McClean, Stephen (2007) Bradykinin-related peptides, including a novel structural variant, (Val(1))-bradykinin, from the skin secretion of Guenther's frog, Hylarana guentheri and their molecular precursors. Peptides, 28 (4). pp. 781-789.
Rao, J. R., Nelson, D. W. A. and McClean, Stephen (2007) The enigma of double-stranded RNA (dsRNA) associated with mushroom virus X (MVX). Current Issues in Molecular Biology, 9. pp. 103-121.
Doherty, Bernadette, Rodriguez, Virginia, Leslie, Julian, McClean, Stephen and Smyth, W. Franklin (2007) An electrospray ionisation tandem mass spectrometric investigation of selected psychoactive pharmaceuticals and its application in drug and metabolite profiling by liquid chromatography/electrospray ionisation tandem mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 21 (13). pp. 2031-2038.
Zhou, Jianwu, Mcclean, Stephen, Thompson, Alan, Zhang, Yang, Shaw, Chris, Rao, Pingfan and Bjourson, AJ (2006) Purification and characterization of novel antimicrobial peptides from the skin secretion of Hylarana guentheri. PEPTIDES, 27 (12). pp. 3077-3084.
Smyth, Franklin, McClean, Stephen, Hack, Catherine, Ramachandran, VN, Doherty, B, Joyce, C, O'Donnell, F, Smyth, Thomas, O'Kane, E and Brooks, P (2006) The characterisation of synthetic and natural-product pharmaceuticals by electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 25 (6). pp. 572-582.
Graham, C, Richter, SC, McClean, Stephen, O'Kane, E, Flatt, Peter and Shaw, C (2006) Histamine-releasing and antimicrobial peptides from the skin secretions of the Dusky Gopher frog, Rana sevosa. PEPTIDES, 27 (6). pp. 1313-1319.
Rustam, Tarick, Mcclean, Stephen, Newcombe, Jane, McFadden, Johnjoe and Eales-Reynolds, Lesley-Jane (2006) Reduced toxicity of lipo-oligosaccharide from a phoP mutant of Neisseria meningitidis: an in vitro demonstration. Journal of Endotoxin Research, 12 (1). pp. 39-46.
Smyth, Franklin, Leslie, Julian, McClean, Stephen, Hannigan, Bernie, McKenna, Hugh, Doherty, B, Joyce, C and O'Kane, E (2006) The characterisation of selected antidepressant drugs using electrospray ionisation with ion trap mass spectrometry and with quadrupole time-of-flight mass spectrometry and their determination by high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20 (11). pp. 1637-1642.
Thompson, Alan Hunter, Bjourson, AJ, Shaw, Chris and McClean, Stephen (2006) Bradykinin-related peptides from Phyllomedusa hypochondrialis azurea: mass spectrometric structural characterisation and cloning of precursor cDNAs. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 20 (24). pp. 3780-3788.
Graham, R. L. J., Graham, C, McClean, Stephen, Chen, T., O'Rourke, M., Hirst, D., Theakston, D. and Shaw, C. (2005) Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers. Biochemical and biophysical research communications, 338 (3). pp. 1587-1592.
Zheng, H, Ojha, PC, McClean, Stephen, Graham, C, Black, ND, Hughes, JG and Shaw, C (2005) A computational approach for peptidomic analysis in taxonomic study of amphibian species. IEEE Tran. on Nanabioscience, 4 (3). pp. 241-247.
Graham, RL, McClean, Stephen, O'Kane, EJ, Theakston, D and Shaw, C (2005) Adenosine in the venoms from viperinae snakes of the genus Bitis: identification and quantitation using LC/MS and CE/MS. Biochemical and biophysical research communications, 333 (1). pp. 88-94.
Graham, C, Irvine, A. E., McClean, Stephen, Richter, S. C., Flatt, Peter and Shaw, C. (2005) Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa. Peptides, 26 (5). pp. 737-743.
Zheng, H, Ojha, PC, McClean, S, Black, ND, Hughes, JG and Shaw, C (2004) Peptidomic pattern analysis and taxonomy of amphibian species. In: Computational and Information Science. Springer, pp. 498-503. ISBN 978-3-540-24127-0
Marenah, L, Shaw, C, Orr, DF, McClean, Stephen, Flatt, Peter and Abdel-Wahab, Yasser (2004) Isolation and characterisation of an unexpected class of insulinotropic peptides in the skin of the frog Agalychnis litodryas. REGULATORY PEPTIDES, 120 (1-3). pp. 33-38.
Marenah, L, McClean, Stephen, Flatt, Peter, Orr, DF, Shaw, C and Abdel-Wahab, Yasser (2004) Novel insulin-releasing peptides in the skin of Phyllomedusa trinitatis frog include 28 amino acid peptide from dermaseptin BIV precursor. PANCREAS, 29 (2). pp. 110-115.
Marenah, L., Flatt, Peter, Orr, D. F., McClean, Stephen, Shaw, C. and Abdel-Wahab, Yasser (2004) Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris. Journal of Endocrinology, 181 (2). pp. 347-354.
Marenah, L, Flatt, Peter, Orr, DF, McClean, Stephen, Shaw, C and Abdel-Wahab, Yasser (2004) Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures. BIOLOGICAL CHEMISTRY, 385 (3-4). pp. 315-321.
Zheng, H, Ojha, PC, McClean, S, Robinson, R, Black, ND, Hughes, JG and Shaw, C (2004) HPLC/ESI-MS Identification of Common Peptides in Amphibian Skin Secretions. In: SET for Britain 2004, the House of Commons, London. .
Chen, TB, Orr, DF, O'Rourke, M, McLynn, C, Bjourson, AJ, McClean, Stephen, Hirst, D, Rao, PF and Shaw, C (2004) Pachymedusa dacnicolor tryptophyllin-1: structural characterization, pharmacological activity and cloning of precursor cDNA. REGULATORY PEPTIDES, 117 (1). pp. 25-32.
Lund, F. T., Orr, D. F., McClean, Stephen, Shaw, S., Lamey, P. J. and Linden, G. J. (2003) Enzyme catabolism of calcitonin gene-related peptide in periodontitis.. Journal of dental research, 82. pp. 570-570.
Gallizia, I, McClean, Stephen and Banat, Ibrahim (2003) Bacterial biodegradation of phenol and 2,4-dichlorophenol. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 78 (9). pp. 959-963.
Chen, TB, Bjourson, AJ, McClean, Stephen, Orr, DF, O'Kane, EJ, Rao, PF and Shaw, C (2003) Cloning of maximakinin precursor cDNAs from Chinese toad, Bombina maxima, venom. PEPTIDES, 24 (6). pp. 853-861.
Zheng, H, Ojha, PC, McClean, S, Black, ND, Hughes, JG and Shaw, C (2003) HPLC/ESI-MS identification of common peptides in amphibian skin secretions. In: ITAB 2003: 4th International IEEE EMBS special topic conference on information technology applications in biomedicaine, Conference proceddings - New Solutions for New Challenges, Birmingham, England. .
Zheng, H, Ojha, PC, McClean, Stephen, Black, ND, Hughes, JG and Shaw, C (2003) Heuristic charge assignment for deconvolution of electrospray ionization mass spectra. Rapid Communications in Mass Spectrometry, 17 (5). pp. 429-436.
Rahman, K. S., Rahman, T. J., McClean, Stephen, Marchant, R and Banat, Ibrahim (2002) Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials. Biotechnology progress, 18 (6). pp. 1277-1281.
Chen, TB, Orr, DF, Bjourson, AJ, McClean, Stephen, O'Rourke, M, Hirst, DG, Rao, PF and Shaw, C (2002) Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis). PEPTIDES, 23 (9). pp. 1547-1555.
Zheng, H, Ojha, PC, McClean, S, Black, ND, Hughes, JG and Shaw, C (2002) Comparison of amphibian skin peptides profiles. In: Genes, Proteins and Computers (GPC) VII conference, Southampton. . 7-7
Zheng, H, Ojha, PC, McClean, S, Black, ND, Hughes, JG and Shaw, C (2002) HPLC/ESI – MS identification of common peptides in amphibian skin secretions. In: Genes, Proteins and Computers (GPC) VII conference, Southampton. . 8-8
Chen, TB, Orr, DF, Bjourson, AJ, McClean, Stephen, O'Rourke, M, Hirst, DG, Rao, PF and Shaw, C (2002) Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 (18). pp. 4693-4700.
McClean, Stephen, Robinson, R. C., Shaw, C. and Smyth, Franklin (2002) Characterisation and determination of indole alkaloids in frog-skin secretions by electrospray ionisation ion trap mass spectrometry. Rapid communications in mass spectrometry : RCM, 16 (5). pp. 346-354.
Zheng, H, McClean, S, Ojha, PC, Black, ND, Hughes, JG and Shaw, C (2001) Analysis And Interpretation Of Complex HPLC/MS Spectra For Drug Discovery. Technology and healthcare, 9 (1-2). pp. 95-97.
Conneely, A, McClean, Stephen, Smyth, Franklin and McMullan, Geoffrey (2001) Study of the mass spectrometric behaviour of phthalocyanine and azo dyes using electrospray ionisation and matrix-assisted laser desorption/ionisation. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 15 (22). pp. 2076-2084.
McClean, Stephen, O'Kane, EJ and Smyth, Franklin (2000) The identification and determination of selected 1,4-benzodiazepines by an optimised capillary electrophoresis - electrospray mass spectrometric method. ELECTROPHORESIS, 21 (7). pp. 1381-1389.
McClean, Stephen, O'Kane, EJ and Smyth, Franklin (2000) Electrospray ionisation-mass spectrometric characterisation of selected anti-psychotic drugs and their detection and determination in human hair samples by liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 740 (2). pp. 141-157.
Smyth, Franklin, McClean, Stephen and Ramachandran, V. N. (2000) A study of the electrospray ionisation of pharmacologically significant 1,4-benzodiazepines and their subsequent fragmentation using an ion-trap mass spectrometer. Rapid communications in mass spectrometry : RCM, 14 (21). pp. 2061-2069.
Smyth, Franklin, McClean, Stephen, O'Kane, EJ, Banat, Ibrahim and McMullan, Geoffrey (1999) Application of electrospray mass spectrometry in the detection and determination of Remazol textile dyes. JOURNAL OF CHROMATOGRAPHY A, 854 (1-2). pp. 259-274.
McClean, Stephen, O'Kane, E, Hillis, J and Smyth, Franklin (1999) Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 838 (1-2). pp. 273-291.
Smyth, Franklin and McClean, Stephen (1998) A critical evaluation of the application of capillary electrophoresis to the detection and determination of 1,4-benzodiazepine tranquilizers in formulations and body materials. ELECTROPHORESIS, 19 (16-17). pp. 2870-2882.
McClean, Stephen, O'Kane, EJ, Coulter, Danny, McLean, S and Smyth, Franklin (1998) Capillary electrophoretic determination of trace metals in hair samples and its comparison with high performance liquid chromatography and atomic absorption spectrometry techniques. Electrophoresis, 19 (1). pp. 11-18.
Smyth, Franklin, Harland, GB, McClean, Stephen, McGrath, G and Oxspring, D (1997) Effect of on-capillary large volume sample stacking on limits of detection in the capillary zone electrophoretic determination of selected drugs, dyes and metal chelates. JOURNAL OF CHROMATOGRAPHY A, 772 (1-2). pp. 161-169.
Harland, G. B., McGrath, G., McClean, Stephen and Smyth, Franklin (1997) Use of large volume sample stacking for improving limits of detection in the capillary electrophoretic determination of selected cations. Analytical Communications, 34 (1). pp. 9-11.
McGrath, G, McClean, Stephen, O'Kane, E, Smyth, Franklin and Tagliaro, F (1996) Study of the capillary zone electrophoretic behaviour of selected drugs, and its comparison with other analytical techniques for their formulation assay. JOURNAL OF CHROMATOGRAPHY A, 735 (1-2). pp. 237-247.
Oxspring, DA, McClean, Stephen, O'Kane, EJ and Smyth, Franklin (1995) Study of the chelation of boron with Azomethine H by differential pulse polarography, liquid chromatography and capillary electrophoresis and its analytical applications. Analytica Chimica Acta, 317 (1-3). pp. 295-301.
McClean, Stephen, MAXWELL, T, RAMACHANDRAN, VN, O'KANE, E and Smyth, Franklin (1994) A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS. ELECTROANALYSIS, 6 (11-12). pp. 996-1003.
McClean, Stephen, O'KANE, E, RAMACHANDRAN, VN and Smyth, Franklin (1994) DIFFERENTIAL-PULSE POLAROGRAPHIC STUDY OF THE DEGRADATION OF H+/K+ ATPASE INHIBITORS SK-AND-F-95601 AND OMEPRAZOLE IN ACIDIC MEDIA AND THE SUBSEQUENT REACTIONS WITH THIOLS. ANALYTICA CHIMICA ACTA, 292 (1-2). pp. 81-89.
O'Kane, EJ, Ramachandran, VN, McClean, Stephen and Smyth, Franklin (1993) Ultraviolet Spectrophotometric, Solvent-Extraction, High-Performance Liquid-Chromatographic and Polarographic Study of the H+. Analyst, 118 (5). pp. 511-516.
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