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Dr Clifford Barnes

Research Associate in 3D Tissue Culture

Brief Profile

Clifford Barnes graduated from the University of Ulster in 2003 with a BSc (Hons) in Biomedical Science and a Diploma in Industrial Science. He completed his PhD in 2007 entitled "Supra-Vital Atomic Force Microscopy of Living Cultured Cells." This work involved creating an imaging environment whereby cells could be imaged in physiological conditions whilst monitoring intracellular ion activity. Using functionalised AFM tip techniques Clifford successfully imaged the locations and bonding strengths of the epidermal growth factor receptor in human lung cancer cells where the over-expression of this important receptor is a characteristic of certain cancers. Clifford then became employed as a research associate under a 3 year contract in the European NanoInteract Project, headed by Professor Howard and Dr George McKerr. He is currently employed as a research associate in work funded by NC3Rs involving 3D cell culture.

Current Research

Clifford’s current interests involve the development of a human breast tissue model using 3D cell culture techniques. Using a range of microscopical techniques Clifford carries out long term imaging of living cells in collagen gels as they form complex structures over a period of days and in some cases weeks.

Dr Clifford Barnes


Dr Clifford Barnes - Publications

Number of items: 11.

Barnes, Clifford, McKerr, George, Soto, A. M., Downes, Stephen, Howard, Vyvyan and Saetzler, Kurt (2014) From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time. PLOS ONE, 9 (4). pp. 1-12.

Speroni, Lucia, Whitt, Gregory S, Xylas, Joanna, Quinn, Kyle P, Jondeau-Cabaton, Adeline, Barnes, Clifford, Georgakoudi, Irene, Sonnenschein, Carlos and Soto, Ana M (2013) Hormonal regulation of epithelial organization in a three-dimensional breast tissue culture model.. Tissue engineering. Part C, Methods, part c.

Park, Margriet V. D. Z., Verharen, Henny W., Zwart, Edwin, Hernandez, Lya G., van Benthem, Jan, Elsaesser, Andreas, Barnes, Clifford, McKerr, George, Howard, Vyvyan, Salvati, Anna, Lynch, Iseult, Dawson, Kenneth A. and de Jong, Wim H. (2011) Genotoxicity evaluation of amorphous silica nanoparticles of different sizes using the micronucleus and the plasmidlacZgene mutation assay. Nanotoxicology, 5 (2). pp. 168-181.

Hazekamp, J, Doherty, S, Elsaesser, A, Barnes, Clifford, O'Hagan, Barry, McKerr, George and Howard, Vyvyan (2011) Focussed ion beam milling at grazing incidence angles.. Journal of microscopy, 242 (1). pp. 104-10.

Elsaesser, Andreas, Barnes, Clifford A, McKerr, George, Salvati, Anna, Lynch, Iseult, Dawson, Kenneth A and Howard, Vyvyan (2011) Quantification of nanoparticle uptake by cells using an unbiased sampling method and electron microscopy.. Nanomedicine (London, England), 6 (7). pp. 1189-98.

McCoy, F, Hurwitz, J, McTavish, N, Paul, I, Barnes, Clifford, O'Hagan, Barry, Odrzywol, K, Murray, J, Longley, D, McKerr, George and Fennell, D A (2010) Obatoclax induces Atg7-dependent autophagy independent of beclin-1 and BAX/BAK. Cell Death and Disease, 1 (12). pp. e108.

Lamers, Edwin, Walboomers, X Frank, Domanski, Maciej, McKerr, George, O'Hagan, Barry, Barnes, Clifford, Peto, Lloyd, Luttge, Regina, Winnubst, Louis A J A, Gardeniers, Han J G E and Jansen, John A (2010) Cryo DualBeam Focused Ion Beam-Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds.. Tissue engineering. Part C, Methods, 17 (1). pp. 1-7.

Park, Margriet VDZ, Annema, Wijtske, Salvati, Anna, Lesniak, Anna, Elsaesser, Andreas, Barnes, Clifford, McKerr, George, Howard, Vyvyan, Lynch, Iseult, Dawson, Kenneth A, Piersma, Aldert H and de Jong, Wim H (2009) In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. TOXICOLOGY AND APPLIED PHARMACOLOGY, 240 (1). pp. 108-116.

Van Hoecke, Karen, Quik, Joris TK, Mankiewicz-Boczek, Joanna, De Schamphelaere, Karel AC, Elsaesser, Andreas, Van der Meeren, Paul, Barnes, Clifford, McKerr, George, Howard, Vyvyan, Van De Meent, Dik, Rydznyski, Konrad, Dawson, Kenneth, Salvati, Anna, Lynch, Iseult, Silversmit, Geert, De Samber, Bjorn, Vincze, Laszlo and Janssen, Colin R (2009) Fate and Effects of CeO2 Nanoparticles in Aquatic Ecotoxicity Tests. Environmental Science Technology, 43 (12). pp. 4537-4546.

Barnes, Clifford, Elsaesser, Andreas, Arkusz, Joanna, Smok, Anna, Palus, Jadwiga, Les?niak, Anna, Salvati, Anna, Hanrahan, John P., Jong, Wim H. de, Dziuba?towska, Elz?bieta, Ste?pnik, Maciej, Rydzyn?ski, Konrad, McKerr, George, Lynch, Iseult, Dawson, Kenneth A. and Howard, Vyvyan (2008) Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity. Nano Letters, 8 (9). pp. 3069.

Barnes, Clifford, O'Hagan, Barry, Howard, Vyvyan and McKerr, G (2007) Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope.. Journal of microscopy, 228 (Pt 2). pp. 185-9.

This list was generated on Wed Jun 28 21:47:24 2017 BST.