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Transcriptional Regulation and Epigenetics
Recombinant DNA Technology
Transgenic animals: generation and use
These lectures and the associated assignment are designed to provide an understanding of the way in which the ability to manipulate whole animal genomes has developed from basic research into early stages of animal development, stem cells and germ cells.
Transgenics can be defined, in it’s broadest terms, as "Gene transfer into intact organisms" (Gordon and Ruddle, 1981). It usually involves the addition of a new gene or the deletion of an existing gene, though more sophisticated manipulations are also now possible.
For additional reading, see for instance:
Classic papers:
Jaenisch R and Mintz, B 1974 Proc. Natl Acad Sci (PNAS) 71:1250
Jaenisch R 1976 PNAS 73:1260
Gordon JW et al, PNAS 77:7380
Costantini F and Lacey, E 1981 Nature 294:92
Gordon JW and Ruddle, FH 1981 Science 214:1244
Wagner EF et al 1983 Cell 35:647
Some recent papers:
Hsu W et al 2001 J. Cell Biol. 155:1055
Igarashi et al 1999 Am J Physiol 277: F599
Elliott JF et al 2003 PNAS 100:13447
Rajewsky K et al J. Clin. Invest. 98:600 [Nice short review of conditional knockout technique]
On the web: Strachan and Read’s Human Molecular Genetics 2 on NIH Bookshelf: